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Mass spectrometry
- In A2/A-Level Chemistry, M+ ion m/z is equal to molecular mass (combine with give empirical formula to deduce molecular formula)
- Identify key fragment peaks to deduce part of the structure of the molecule
Fragment ion | M/z value |
CH3+ | 17 |
CH2CH3+ | 29 |
CH2CH2CH3+ or CH3CO+ | 43 |
CH2CH2CH2CH3+ | 57 |
OH+ | 17 |
C6H4CH3+ or C6H3(CH2)2+ (aromatic) | 91 |
Infrared spectroscopy
- Use characteristic adsorptions to identify functional groups in the molecule
13C-NMR
- In A2/A-Level Chemistry, Number of peaks --> number of different carbon environments
- Chemical shift --> type of carbon environment
1H-NMR
- In A2/A-Level Chemistry, Number of peaks --> number of different hydrogen environments
- Chemical shift --> type of hydrogen environment
- Splitting pattern --> n+1 hydrogens on the adjacent carbon
- Relative peak area --> number of hydrogens in each environment
- D2O--> Peaks that disappear are either –OH or –NH (because the deuterium exchanges with the present hydrogen atom
That's the end~