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HKDSE Biology - Microscope 🔬

Compulsory Part I Cells and Molecules of Life

· HKDSE,Biology,Microscope,Light microscope,Electron microscope
  • Appreciate the contribution of the technological development of the microscope to the discovery of cells.
  • Prepare temporary mounts of specimens for examination, and make observations and drawings under a light microscope.
  • Identify cell organelles as seen under light and electron microscopes.
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Why cell can be discovered 🤔 ❓ ❔

  • Invention of microscope make the discovery of cell become achievable.
  • The scientist who first discovered cell is Robert Hooke. 👨🏻‍🔬

That's the microscope Robert Hooke utilised to discover cell 👇🏻

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How to make observation under light microscope 😲❓ ❔

1. Preparation of temporary mounts

  • Sectioning, staining and mounting are the process for preparing a temporary mounts. 
  • 1️⃣ A drop of water is placed in the centre of the slide. 
  • 2️⃣ Position the sample on the drop of water.
  • 3️⃣ Place the cover slip slowly on top of the sample to avoid the occurrence of air bubbles.
  • 4️⃣ Excessive water is removed from the slid with a paper towel.
  • 🔺 Staining the sample with iodine, methylene blue and crystal violet can achieve a clearer observation. 

2. Observation under light microscope

  • 1️⃣ Moving the stage down to the lowest position.
  • 2️⃣ Place the slide onto the stage and hold in position with the clip.
  • 3️⃣ Select the lens with the lowest magnification.
  • 4️⃣ Turing of coarse adjustment knob till you can see the cells.
  • 5️⃣ Turing of fine adjustment knob till you can see the cells clearly.
  • 6️⃣ Using lens with higher magnification with see the cells with larger magnification that more details of the cells can be observed . 

3. Drawing of the cell under observation 

In HKDSE Biology examination, you have to know how to draw the organelle you observed under light microscope with label ❗️ ❕

Drawing of animal cell

👆🏻This is the drawing of animal cell with labels.

Drawing of animal cell

👆🏻This is the drawing of plant cell with labels.

Identification of organelles under light microscope and electron microscope 💡

In HKDSE Biology examination, you will be given photo as shown and you have to identify the organelle one by one ❗️ ❕

Animal cell and plant cell under electron microscope

👆🏻These are the image of animal cell and plant cell under electron microscope.

That's the end of this topic 👏🏻

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Written by Bella | HKDSE Biology Specialist @ TUTTEE

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R eferences

  1. Rhoads, D. (2020, March 18). History of Cell Biology. Retrieved from https://bitesizebio.com/166/history-of-cell-biology/
  2. Microscope Slides Preparation - Styles and Techniques. (n.d.). Retrieved from https://www.microscopemaster.com/microscope-slides.html 
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