GCE CIE Biology - Microscopy
GCE CIE - Chapter 1 Cell Structure
1.1.d Explain and distinguish between resolution and magnification, with reference to light microscopy and electron microscopy
1.1.e Calculate actual sizes of specimens from drawings, photomicrographs and electron micrographs
RESOLUTION VS MAGNIFICATION:
Resolution: The ability to distinguish between two separate objects that are very close together. The higher the resolution, the greater the detail of the image.
Magnification: The number of times larger an image is compared with the real size of the object.
LIGHT VS ELECTRON MICROSCOPES:
Here are somethings to keep in mind when comparing light and electron microscopes for a GCE CIE Biology exam:
🤯 As you can see from the table above, using an electron microscope will produce a more detailed photo of the microscopic object because it has a nearly 400x better resolution than using a light microscope!
CALCULATING ACTUAL SIZE OF MICROSCOPIC OBJECTS:
As explained in another article, to calculate the magnification, we use the following triangle:
Therefore, to calculate actual size, we simply rearrange the formula to:
Actual size (A) = Image Size with Ruler (I) ÷ Magnification (M)
⚠️⚠️The above triangle is extremely useful! Remembering it will come in handy for other calculations (such as calculating magnification) should a question on the above appear in a GCE CIE Biology exam!
That's it! Till next time! 🙌
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Written by Aayushi | GCE CIE Biology Specialist @ TUTTEE
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